nested primers meaning in English
嵌套引物
Examples
- In order to identifiy the virus further , a set of double nested primers for canine coronavirus was selected . the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl , ccvf2 - ccvr2 . the first is a pair of outer primer , and can amplify a fragement of 1086bp . the second is a pair of inner primer . and can amplify a fragement of 515bp . using the nested primers , many ccv strains can be identificated including k378 , insave - l , ccv 1 - 71 etc . synthesizing this set of primers , we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr , and all of them respectively gained two target fragements of 1086bp and 515bp , but the control cell did not
合成该套式引物，选择大熊猫原代病料和病毒各代细胞培养物，经套式( nested ) rt一pcr扩增，可得到一与设计值5巧bp相符的dna片段，经bst一xl ( 590 ， 1110 )酶切鉴定，证明该扩增片断为特异性片段;回收大熊猫肝组织原代病料和细胞培养物第2 、 3 、 29代的ccvfz一ccvrz扩增片段，纯化，送生物公司测序。